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(a) Effect of serum deprivation on TRPC6 cell surface abundance and the regulation by soluble Klotho. HEK cells expressing TRPC6 were cultured in serum deprived or serum containing media for 24h and further incubated with soluble Klotho (200pM) for 2h before subjecting to biotinylation assay. The abundance of tubulin in lysates served as a loading control.

There a lot of colour on the eyes and lips this season. That in itself is optimistic. And I think women now, on the streets, are wearing colour, which they weren in the past few years. But Christopher Nolan, even in his earlier, more modestly budgeted films, has never been content with the small scale. His imagination is large; his eye seeks out wide, sweeping vistas; and if he believes in anything, it is ambition. When her father is recruited for a secret NASA mission to search for a habitable new planet, Murph is devastated by his departure.

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